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H9250

Sigma-Aldrich

Histone from calf thymus

Type II-A, lyophilized powder

Synonym(s):

histone type VIII-S

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About This Item

CAS Number:
Beilstein/REAXYS Number:
1895465
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.51

biological source

bovine (calf) thymus

type

Type II-A

form

lyophilized powder

application(s)

cell analysis

storage temp.

2-8°C

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General description

Histones are octameric proteins containing H3, H4, H2A and H2B. It is the core protein around which DNA is wound forming the nucleosome structure. The core histone proteins contain globular domains. Each protein is composed of basic amino acids at its N-terminal. The H2A has an extra amino acid at its C-terminal end forming a histone tail. The nucleosome structure is stabilized by the linker histones during chromatin condensation.

Application

Histone from calf thymus has been used:
  • in methyltransferase reaction mixture for methyltransferase assay
  • in in vitro binding assay, to explore the potential binding of the MORF4 (mortality factor on chromosome 4) - related gene on chromosome 15 (MRG15) chromo domain with histone
  • in in vitro coculture assays, to stimulate liver resident Kupffer cells

Biochem/physiol Actions

The core histones are involved in histone-histone interactions and maintains the organization of the nucleosomal DNA. The histone tail helps in forming higher-order nucleosomal structures. The overall structure and action of chromatin fiber is regulated by histone modifications.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Preparation Note

Unfractionated whole histone

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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