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F1804

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

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Synonym(s):
flag antibody, Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

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This Item
F2426F9291A2220
conjugate

unconjugated

conjugate

-

conjugate

biotin conjugate

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody form

-

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

form

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

-

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Browse additional application references in our FLAG® Literature portal.

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins
Aparna A
Nucleic Acids Research, 45(7), 4189-4201 (2017)
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Monika Srivastava et al.
Nature communications, 6, 6253-6253 (2015-02-24)
Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances
HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
Yalan Liu
Virology, 154-154 (2016)

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