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P6282

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Synonym(s):

PLL HBr

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75

product name

Poly-L-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

70,000-150,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

solubility

H2O: soluble 50 mL/vial, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.

Biochem/physiol Actions

Poly-L-lysine serves as a pre-coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Does NAD (P) H oxidase-derived H 2 O 2 participate in hypotonicity-induced insulin release by activating VRAC in beta-cells?
Crutzen R, et al.
Pfluegers Archiv, 463(2), 377-390 (2012)
Rahul Munshi et al.
Frontiers in neuroscience, 12, 560-560 (2018-08-30)
Determining the role and necessity of specific neurons in a network calls for precisely timed, reversible removal of these neurons from the circuit via remotely triggered transient silencing. Previously, we have shown that alternating magnetic field mediated heating of magnetic
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner, et al.
PLoS ONE, 17(7) (2022)
Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo
June Seok Heo, et al,
BioMed Research International (2016)
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Faussner A, Deininger MM, Weber C, Steffens S
Journal of Separation Science, 17(7), e0260173-e0260173 (2022)

Articles

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocols

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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