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MilliporeSigma

P1524

Sigma-Aldrich

Poly-L-lysine hydrobromide

suitable for cell culture, Mol wt ≥300,000

Synonym(s):

L-Lysine homopolymer hydrobromide

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25 MG
$74.80
100 MG
$221.00
500 MG
$649.44
1 G
$941.60

$74.80


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Available to ship onJanuary 09, 2025Details


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25 MG
$74.80
100 MG
$221.00
500 MG
$649.44
1 G
$941.60

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26

$74.80


Availability

Available to ship onJanuary 09, 2025Details


Request a Bulk Order

product name

Poly-L-lysine hydrobromide, mol wt ≥300,000

form

powder

Quality Level

mol wt

≥300,000

technique(s)

cell culture | mammalian: suitable

color

white to off-white

application(s)

cell analysis

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

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This Item
P7890P6516P1399
technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

white to off-white

color

white to off-white

color

white to off-white

color

white to off-white

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

mol wt

≥300,000

mol wt

15,000-30,000 by viscosity

mol wt

≤15,000 by MALLS, 4,000-15,000 by viscosity

mol wt

150,000-300,000

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. It has also been used as a transfection agent for efficient labeling[1][2] and to facilitate endocytosis.[2] Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

Poly-L-lysine hydrobromide has a molecular weight >300,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Analysis Note

Molecular weight based on viscosity and is also assayed by MALLS.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Joshua Guild et al.
Molecular biology of the cell, 28(14), 1975-1983 (2017-05-05)
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Amsalem Y, et al.
Circulation, 116 (2007)
In vivo tracking of mesenchymal stem cells labeled with a novel chitosan-coated superparamagnetic iron oxide nanoparticles using 3.0 T MRI
Reddy AM, et al.
Journal of Korean Medical Science, 25(2), 211-219 (2010)
Miroslaw Janowski et al.
PloS one, 9(2), e97631-e97631 (2014-06-12)
The purpose of the study was to evaluate the long-term clinical tracking of magnetically labeled stem cells after intracerebroventricular transplantation as well as to investigate in vitro feasibility for magnetic guidance of cell therapy within large fluid compartments. After approval
Robert Andrews et al.
PloS one, 2(6), e493-e493 (2007-06-07)
Endocytosis is involved in the regulation of many cellular events, including signalling, cell migration, and cell polarity. To begin to investigate roles for endocytosis in early C. elegans development, we examined the distribution and dynamics of early endosomes (EEs) in

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