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P6407

Sigma-Aldrich

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Synonym(s):

PDL

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About This Item

Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.75

product name

Poly-D-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioReagent
BioXtra

form

lyophilized powder

mol wt

70,000-150,000

packaging

pkg of 10 X 5 mg
pkg of 5 mg

concentration

0.016—0.032 mmol lysine

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

<10% water (by Karl Fischer)

solubility

H2O: soluble 50 mg/mL, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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application

Poly-D-lysine hydrobromide has been used:

  • as a coating for plates used in culturing rat glial precursor cells (RGPCS).
  • in coating flasks and plates in culturing tanycytes
  • in preparing the surface of coverslips for facilitating cell attachment
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Features and Benefits

  • Sterilized using gamma (γ) radiation.
  • Sterile Poly-D-Lysine solutions can be stored for three years when stored at 2-8˚C.

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 70,000-150,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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