P6407
Poly-ᴅ-Lysine Hydrobromide
synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra
Synonym(s):
PDL
About This Item
Recommended Products
product name
Poly-D-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
biological source
synthetic (organic)
Quality Level
sterility
γ-irradiated
product line
BioReagent
BioXtra
form
lyophilized powder
mol wt
70,000-150,000
packaging
pkg of 10 X 5 mg
pkg of 5 mg
concentration
0.016—0.032 mmol lysine
technique(s)
cell culture | mammalian: suitable
surface coverage
4 μg/cm2
impurities
<10% water (by Karl Fischer)
solubility
H2O: soluble 50 mg/mL, clear, colorless
shipped in
ambient
storage temp.
−20°C
SMILES string
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key
MEXAGTSTSPYCEP-UHFFFAOYSA-N
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Related Categories
application
- as a coating for plates used in culturing rat glial precursor cells (RGPCS).
- in coating flasks and plates in culturing tanycytes
- in preparing the surface of coverslips for facilitating cell attachment
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
Biochem/physiol Actions
Features and Benefits
- Sterilized using gamma (γ) radiation.
- Sterile Poly-D-Lysine solutions can be stored for three years when stored at 2-8˚C.
Components
Caution
Preparation Note
comparable product
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Protocols
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
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