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127-2.5

Sigma-Aldrich

Poly-D-Lysine & Laminin (2.5 ML)

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NACRES:
NA.75

form

liquid

Quality Level

shelf life

1 yr

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

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This Item
A-003-MCLS354232CLS3841
vibrant-m

127-2.5

Poly-D-Lysine & Laminin (2.5 ML)

vibrant-m

A-003-M

Poly-D-Lysine solution, 1.0 mg/mL

vibrant-m

CLS354232

Corning® Laminin

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

form

liquid

form

liquid

form

-

form

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

shelf life

1 yr

shelf life

-

shelf life

-

shelf life

-

General description

Poly-D-Lysine (PDL) and Laminin solution is ideal for use in the coating protocol for cell culture surfaces. PDL Laminin coating is extensively used for neural cultures. It is known to enhance cell adhesion and cell maturation during in vitro cell culture. Poly-D-Lysine and Laminin solution is prepared in phosphate-buffered saline and sterile filtered. It is used to coat tissue culture ware to culture surface ratio of 0.25 ml/cm2. Tissue culture ware is coated at room temperature for one hour and carefully aspirate the solution. After wash two times with PBS, coated tissue culture ware may be used immediately or air-dried and stored at 4oC for up to one week.

Application

Poly-D-Lysine & Laminin (2.5 ML) has been used in vitro as inductive agents to stimulate Y79 cells to express both neuronal and glial characteristics.It has been used to coat culture plates for retinal ganglion cell culture.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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