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Key Documents

127-2.5

Sigma-Aldrich

Poly-D-Lysine & Laminin (2.5 ML)

Synonym(s):

Collagen IV from human placenta

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

form

liquid

Quality Level

100

shelf life

1 yr

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Poly-D-Lysine (PDL) and Laminin solution is ideal for use in the coating protocol for cell culture surfaces. PDL Laminin coating is extensively used for neural cultures. It is known to enhance cell adhesion and cell maturation during in vitro cell culture. Poly-D-Lysine and Laminin solution is prepared in phosphate-buffered saline and sterile filtered. It is used to coat tissue culture ware to culture surface ratio of 0.25 ml/cm2. Tissue culture ware is coated at room temperature for one hour and carefully aspirate the solution. After wash two times with PBS, coated tissue culture ware may be used immediately or air-dried and stored at 4oC for up to one week.

Application

Poly-D-Lysine & Laminin (2.5 ML) has been used in vitro as inductive agents to stimulate Y79 cells to express both neuronal and glial characteristics.It has been used to coat culture plates for retinal ganglion cell culture.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Tombran-Tink et al.
Investigative ophthalmology & visual science, 30(8), 1700-1707 (1989-08-01)
Tumor cells can be induced to differentiate in vitro by biochemical manipulation of their culture environment. In the studies described here, the effects of medium conditioned by human retinal pigmented epithelial (RPE) cells on Y79 human retinoblastoma cells have been
The protective role of sulforaphane and Homer1a in retinal ischemia-reperfusion injury: Unraveling the neuroprotective interplay
Saadh MJ, et al.
Basic Life Sciences (2023)
A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro
Stil A, et al.
Frontiers in Cellular Neuroscience, 17, 1212097-1212097 (2023)
A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro
Aurelie Stil, et al.
Frontiers in Cellular Neuroscience (2023)
Jing-Wen Yu et al.
Journal of molecular neuroscience : MN, 60(4), 486-497 (2016-08-31)
Bone marrow-derived mesenchymal stem cells (MSCs) are the ideal transplanted cells of cellular therapy for promoting neuroprotection and neurorestoration. However, the optimization of transplanted cells and the improvement of microenvironment around implanted cells are still two critical challenges for enhancing
View All Related Papers

Protocols

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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