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Laminin, mouse purified

Laminins are the major noncollagenous constituent of basement membranes. This purified mouse laminin was isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a mouse tumor that produces large amounts of basement membranes.

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eCl@ss:
32160405
NACRES:
NA.75

biological source

mouse

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

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This Item
AG56PSAB4200719SAB4200236
form

liquid

form

liquid

form

buffered aqueous solution

form

buffered aqueous solution

concentration

1 mg/mL

concentration

0.25 mg/mL

concentration

~1.0 mg/mL

concentration

~1.0 mg/mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, electron microscopy: suitable, immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, immunofluorescence: suitable, immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections.

technique(s)

immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable, western blot: 0.1-0.2 μg/mL using whole extracts of human HeLa cells

NCBI accession no.

NM_002291.1

NCBI accession no.

NM_002291.1

NCBI accession no.

-

NCBI accession no.

-

UniProt accession no.

P07942

UniProt accession no.

P07942

UniProt accession no.

P25391

UniProt accession no.

P02545

General description

Product Source: Isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma
Specificity:Molecular weight = 850 kD; 3 chains expressed as two major bands at 200 and 400 kD on SDS-PAGE.

Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.

Application

Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue. The optimal laminin concentration may vary with the cell type and has to be determined experimentally.

Physical form

Purified from EHS sarcoma according to the method of Timpl, et al.
Purified protein. Liquid; in 0.05 M Tris-HCl, pH 7.4, containing 0.15 M NaCl.

No preservatives.

Storage and Stability

Store at -20°C for up to six months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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