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L6274

Sigma-Aldrich

Laminin from human placenta

liquid, BioReagent, suitable for cell culture

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Synonym(s):
Cell culture grade laminin, Human Laminin
CAS Number:
MDL number:

biological source

human placenta

Quality Level

product line

BioReagent

form

liquid

concentration

0.5 mg/mL in Tris-buffered saline

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LAMB1(3912)

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This Item
L2020AG56PL4544
vibrant-m

L6274

Laminin from human placenta

vibrant-m

L4544

Laminin from human fibroblasts

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

human placenta

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

biological source

human

biological source

human fibroblasts

form

liquid

form

aqueous solution

form

liquid

form

liquid

Gene Information

human ... LAMB1(3912)

Gene Information

mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782)

Gene Information

human ... LAMB1(3912)

Gene Information

human ... LAMB1(3912)

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C, −70°C

storage temp.

−70°C

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.

Caution

It is recommended to store this product at -70°C, where it will remain stable for two years.

Preparation Note

This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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