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L4544

Sigma-Aldrich

Laminin from human fibroblasts

cell culture derived, liquid, sterile-filtered

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MDL number:
NACRES:
NA.75

biological source

human fibroblasts

Quality Level

sterility

sterile-filtered

form

liquid

packaging

pkg of 100 μL

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LAMB1(3912)

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This Item
L6274L2020F0556
sterility

sterile-filtered

sterility

-

sterility

-

sterility

sterile-filtered

form

liquid

form

liquid

form

aqueous solution

form

solution

packaging

pkg of 100 μL

packaging

-

packaging

pkg of 1 mg

packaging

pkg of 100 μL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

surface coverage

1‑2 μg/cm2

surface coverage

1‑2 μg/cm2

surface coverage

-

Application

Laminin from human fibroblasts has been used:
  • in coating six-well plates for wound healing assay
  • as a substrate in cell adhesion and spreading assay
  • for differentiation of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) toward dopaminergic neurons
  • in corneal endothelial cell wound healing (migration) assay and corneal endothelial cell barrier assay

Laminin from human fibroblasts is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is produced by human fibroblasts and epithelial cells in a co-culture system and then purified biochemically.

Caution

It is recommended to store this product at -70°C, where it will remain stable for two years.

Preparation Note

This product is supplied at a concentration of 0.5 mg/mL in TBS. Thaw this solution slowly before use at 2-8°C to avoid gel formation. If at any time prior to that, the laminin is allowed to warm up prematurely, gelling can occur. Moreover, even allowing it to be stored at -20C can induce gelling or significantly reduce its activity or both." For use as a coating, dilute in a HBSS, coat culture surface with a minimal volume and incubate at 37°C for 1-2 hours. Wash 3 times with HBBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Protocols

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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