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SRP3186

Sigma-Aldrich

Vitronectin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

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Synonym(s):
Epiboin, S-Protein, Serum-spreading factor, V75, VTN
CAS Number:
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≤0.5 μg/mL EC50

mol wt

52.2 kDa (calculated)

packaging

pkg of 250 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

solubility

water: soluble

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VTN(7448)

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1 of 4

This Item
CC0805051V0132
vibrant-m

SRP3186

Vitronectin human

vibrant-m

CC080

Vitronectin, Human Purified Protein

vibrant-m

5051

Vitronectin

vibrant-m

V0132

Vitronectin from rat plasma

biological source

human

biological source

human

biological source

human plasma

biological source

rat plasma

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

>95%

assay

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

-

recombinant

-

mol wt

52.2 kDa (calculated)

mol wt

-

mol wt

75 kDa

mol wt

75 kDa

General description

Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers.The vitronectin gene consists of approximately 4.5–5 kb base pairs with eight exons and seven introns.
Recombinant human Vitronectin is a protein consisting of 459 amino acids. It exists in two forms: a single chain and a clipped form consisting of two chains held together by disulfide bonds resulting from an endogenous cleavage. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media.

Application

Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.

Biochem/physiol Actions

The multimeric vitronectin protein can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin αvβ3 enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. It is also involved in wound repair and controls complement system as wel. Moreover, vitronectin functions as a marker of profibrotic activity in liver, heart and kidney.

Features and Benefits

  • Human-derived vitronectin
  • Produced through recombinant expression in HEK 293 cells
  • Low endotoxin levels

Sequence

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Physical form

Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

Under reducing conditions, the single chain migrates at an apparent molecular mass of 75kDa on SDS-PAGE, and the clipped form migrates at molecular masses of 65kDa and 10kDa.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Denise Salzig et al.
Stem cells international, 2016, 5246584-5246584 (2016-03-24)
The manufacture of human mesenchymal stem cells (hMSCs) for clinical applications requires an appropriate growth surface and an optimized, preferably chemically defined medium (CDM) for expansion. We investigated a new protein/peptide-free CDM that supports the adhesion, growth, and detachment of
Distribution of vitronectin mRNA during murine development.
Seiffert D, et al.
Developmental Dynamics, 203, 71-79 (1995)
Vitronectin significantly influences prognosis in osteosarcoma.
Shi K, et al.
International Journal of Clinical and Experimental Pathology, 8, 11364-11371 (2015)
Stefan R Braam et al.
Stem cells (Dayton, Ohio), 26(9), 2257-2265 (2008-07-05)
Defined growth conditions are essential for many applications of human embryonic stem cells (hESC). Most defined media are presently used in combination with Matrigel, a partially defined extracellular matrix (ECM) extract from mouse sarcoma. Here, we defined ECM requirements of
Degradation of distinct forms of multimeric vitronectin by human fibroblasts.
Wilkins-Port CE and McKeown-Longo PJ
Biochimica et Biophysica Acta, 1404, 353-366 (1998)

Protocols

Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. This protocol is for coating a 96-well plate and other surfaces.

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