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MilliporeSigma

F1141

Sigma-Aldrich

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

Synonym(s):

Fibronectin

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About This Item

EC Number:
289-149-2
MDL number:
MFCD00131062
UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75

Key Documents

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Product Name

Fibronectin bovine plasma, solution, sterile-filtered, BioReagent, suitable for cell culture

biological source

bovine plasma

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

UniProt accession no.

P07589

binding specificity

Peptide Source: Collagen

Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Gene Information

bovine ... FN1(280794)

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General description

Fibronectin is a ubiquitous protein of the extracellular matrix. It is a mosaic protein, that is made up of three types of repeating modular consensus amino acid sequences. Fibronectins belongs to a class of high-molecular-weight glycoproteins. Cellular fibronectin is expressed by fibroblasts and plasma fibronectin is usually liberated by hepatocytes.

Application

Fibronectin (FN) bovine plasma has been used in:
  • fibronectin and adsorption analysis (ELISA (enzyme-linked immunosorbent assay))
  • transwell migration
  • fibronectin assay

Biochem/physiol Actions

Fibronectins participate in cell adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Other Notes

Product F1141, Fibronectin from bovine plasma is a solution in 0.05 M TBS pH 7.5.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000489678
0000489678
0000481930
0000481930
0000438510

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Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells
Khang D, et al.
Biomaterials, 33(26), 5997-6007 (2012)
CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms
Fang W B, et al.
The Journal of biological chemistry, jbc-M112 (2012)
FIBRONECTIN AUTOANTIBODIES
Autoantibodies, 221-251 (2007)
Enhanced fibronectin adsorption on carbon nanotube/poly (carbonate) urethane: independent role of surface nano-roughness and associated surface energy
Khang D, et al.
Biomaterials, 28(32), 4756-4768 (2007)
Tests to predict preeclampsia
Chesley's Hypertensive Disorders in Pregnancy, 221-251 (2015)
View All Related Papers

Articles

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Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

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Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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