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PluriSTEM-XF® Recombinant Vitronectin

Human pluripotent stem cell culture.

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Synonym(s):
S-protein, Serum-spreading factor, V75
eCl@ss:
32160405
NACRES:
NA.75

biological source

Escherichia coli
human

Quality Level

Assay

≥92% (SDS-PAGE)

form

liquid

manufacturer/tradename

PluriSTEM®

concentration

0.5 mg/mL

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell culture | stem cell: suitable

input

sample type: human embryonic stem cell(s)

UniProt accession no.

shipped in

dry ice

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This Item
SRP3186V0132V9881
Vitronectin human recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3186

Vitronectin human

assay

≥92% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

-

assay

-

form

liquid

form

lyophilized

form

lyophilized powder

form

lyophilized powder

concentration

0.5 mg/mL

concentration

-

concentration

-

concentration

-

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P04004

UniProt accession no.

P04004

UniProt accession no.

Q3KR94

UniProt accession no.

Q3ZBS7

General description

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Application

Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion
Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Quality

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Physical form

Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.

Storage and Stability

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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