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Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture

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CAS Number:
MDL number:

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

Quality Level

product line



aqueous solution

mol wt

A subunit 400 kDa
B1 subunit 210 kDa
B2 subunit 200 kDa


pkg of 1 mg


1-2 mg/mL in Tris-buffered saline


cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2


Microbial Contamination, passes test

NCBI accession no.

UniProt accession no.

Binding Specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.


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This Item
Laminin from mouse Engelbreth-Holm-Swarm (EHS) sarcoma





aqueous solution




lyophilized powder



mol wt

A subunit 400 kDa, B1 subunit 210 kDa, B2 subunit 200 kDa

mol wt


mol wt


mol wt

900 kDa


pkg of 1 mg




glass bottle of 1 vial


pkg of 1 mg (2 ml)


1-2 mg/mL in Tris-buffered saline


0.5 mg/mL in Tris-buffered saline






cell culture | mammalian: suitable


cell culture | mammalian: suitable


cell culture | mammalian: suitable


cell culture | mammalian: suitable

General description

Laminins are non-collagenous adhesive glycoproteins with a substantial molecular weight. They are heterotrimeric proteins with three disulfide-linked polypeptides known as the α, β, and γ chains. The multiple polypeptide domains of laminins can bind to multiple cell surface receptors, including several integrin dimers, dystroglycan and syndecans, and other ECM components. This extracellular matrix multidomain trimeric glycoprotein is the main component of basal lamina that supports adhesion, proliferation and differentiation.


Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:
  • insulin-producing cell (IPC) differentiation.
  • Infection inhibition assays.
  • Proliferation Assay.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.


Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.


It is recommended to store this product in working aliquots at -20°C.

Preparation Note

This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

related product

Product No.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor.
Thepparit C and Smith DR.
Journal of Virology, 78(22), 12647-12656 (2004)
Tobias Bergström et al.
Biochimica et biophysica acta, 1840(8), 2526-2532 (2014-01-28)
It is becoming increasingly apparent that the extracellular matrix acts as an important regulator of the neural stem niche. Previously we found that neural stem and progenitor cells (NSPCs) derived from the early postnatal subventricular zone of mice adhere to
Agnese Loda et al.
Nature communications, 8(1), 690-690 (2017-09-28)
Xist is indispensable for X chromosome inactivation. However, how Xist RNA directs chromosome-wide silencing and why some regions are more efficiently silenced than others remains unknown. Here, we explore the function of Xist by inducing ectopic Xist expression from multiple
Axonal pathfinding: Extracellular matrix role
Letourneau, P
Encyclopedia of Neuroscience, 1, 1139-1145 (2009)
Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK.
Lin HY, et al.
Journal of Biomedical Science, 17:56 (2010)


The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

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Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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