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11243217001

Roche

Laminin

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

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Synonym(s):
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
CAS Number:
MDL number:

biological source

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

Quality Level

sterility

sterile; sterile-filtered

Assay

≥90% (SDS-PAGE)

form

solution

mol wt

900 kDa

packaging

pkg of 1 mg (2 ml)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Lamb2(16779)

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This Item
L2020CC145AG56P
Laminin from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

Roche

11243217001

Laminin

Rat Laminin-5

Sigma-Aldrich

CC145

Rat Laminin-5

sterility

sterile; sterile-filtered

sterility

-

sterility

-

sterility

-

assay

≥90% (SDS-PAGE)

assay

-

assay

>95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

solution

form

aqueous solution

form

liquid

form

liquid

mol wt

900 kDa

mol wt

A subunit 400 kDa, B1 subunit 210 kDa, B2 subunit 200 kDa

mol wt

-

mol wt

-

packaging

pkg of 1 mg (2 ml)

packaging

pkg of 1 mg

packaging

-

packaging

-

General description

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.

Application

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Quality

Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)

Sequence

Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.

Physical form

Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane

Preparation Note

Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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Protocols

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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