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C2874

Sigma-Aldrich

CryoStor® cell cryopreservation media

CS10

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Synonym(s):
cell freezing medium
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

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This Item
C9249C6295C6039
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

200

General description

The CryoStor CS2, CS5, and CS10 family of preservation solutions represents the next generation of cryopreservation media. Designed to prepare and preserve cells in ultra low temperature environments (–80 to –196 °C), CryoStor media provide a safe, protective environment for cells and tissues during thefreezing, storage, and thawing process. Through modulating the cellular biochemical response to the cryopreservation process, these media provide enhanced cell viability and functionality, while eliminating the need to include serum, proteins, or high levels of cytotoxic agents.

CryoStor CS2, CS5, and CS10 are a series of cell specific, optimized preservation media, uniquely formulated to address the molecular biological aspects of cells during the cryopreservation process; thereby, directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

These media are recommended for the preservation of stem cells, hepatocytes, tissue samples, and other extremely sensitive cell types.

Application

CryoStor® cell cryopreservation media has been used in:
  • the preservation of T cells
  • the preservation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
  • hiPSC-derived cardiac progenitors (hiPSC-CPs)
  • as a component of cryoprotective solution composed of bovine albumin

CryoStor, a series of cell-specific, optimized preservation media, is uniquely formulated to address the molecularbiological aspects of cells during the cryopreservation process thereby directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

Other Notes

Formulation contains 10% DMSO.

Legal Information

CryoStor is a registered trademark of BioLife Solutions, Inc.

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Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Huyen Thi Lam Nguyen et al.
STAR protocols, 1(2) (2020-10-13)
Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor
Susie Barbeau et al.
Stem cell research, 49, 102106-102106 (2020-12-30)
Congenital myasthenic syndromes (CMS) are a class of inherited disorders affecting the neuromuscular junction, a synapse whose activity is essential for movement. CMS with acetylcholinesterase (AChE) deficiency are caused by mutations in COLQ, a collagen that anchors AChE in the
Physical events occurring during the cryopreservation of immortalized human T cells
Meneghel J, et al.
PLoS ONE, 14(5), e0217304-e0217304 (2019)
In vivo maturation of human induced pluripotent stem cell-derived cardiomyocytes in neonatal and adult rat hearts
Kadota S, et al.
Stem Cell Reports, 8(2), 278-289 (2017)
The impact of varying cooling and thawing rates on the quality of cryopreserved human peripheral blood T cells
Baboo J, et al.
Scientific reports, 9(1), 3417-3417 (2019)

Articles

Overview of good cell banking practices for cell line cryopreservation purposes.

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Protocols

Cryopreservation efficacy which includes post-thaw recovery, viability, and functionality is of importance to both research and clinical applications. The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis.

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

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