T9424
TRI Reagent®
For processing tissues, cells cultured in monolayer or cell pellets
Recommended Products
Quality Level
usage
mL sufficient for 107 cells
mL sufficient for 100 mg tissue (or)
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.
Application
- in the isolation of total RNA from various tissue samples and cells
- for the lysis of monocytes
- to isolate cytoplasmic RNA from SARS-CoV infected Vero cells
- to isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus
- to prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine
The RNA, DNA and protein that are isolated using TRI reagent® can further be used for downstream applications, such as cloning, PCR, RT-PCR, Northern blots, mRNA isolation, in vitro translation, RNase protection assay, restriction enzyme digestion, Southern blots, SDS-PAGE and western blots.
Features and Benefits
Multi-purpose: TRI Reagent® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples.
Efficient: TRI reagent® gives better yields than traditional guanidine thiocyanate/cesium chloride methods. The whole process of RNA extraction starting with fresh tissue or cells can be completed in less than one hour.
- Easily scalable RNA isolation
- Works with many sources: human, plant, yeast, bacterial, or viral
- Better yields than traditional guanidine thiocyanate/cesium chloride methods
Legal Information
related product
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
supp_hazards
wgk_germany
WGK 2
flash_point_f
174.2 °F
flash_point_c
79 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Protocols
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Related Content
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service