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C3124

Sigma-Aldrich

CryoStor® cell cryopreservation media

CS2

Synonym(s):

Cell Cryopreservation Solution, Cryopreservation Media

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

100

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

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General description

The CryoStor CS2, CS5, and CS10 family of preservation solutions represents the next generation of cryopreservation media. Designed to prepare and preserve cells in ultra low temperature environments (-80 to -196 °C), CryoStor media provide a safe, protective environment for cells and tissues during thefreezing, storage, and thawing process. Through modulating the cellular biochemical response to the cryopreservation process, these media provide enhanced cell viability and functionality, while eliminating the need to include serum, proteins, or high levels of cytotoxic agents.

CryoStor CS2, CS5, and CS10 are a series of cell specific, optimized preservation media, uniquely formulated to address the molecular biological aspects of cells during the cryopreservation process; thereby, directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

These media are recommended for the preservation of stem cells, hepatocytes, tissue samples, and other extremely sensitive cell types.

Application

CryoStor CS2 is formulated to contain 2% dimethyl sulfoxide (DMSO). Suggested when reducing DMSO is of primary concern.

Cryostor® Cryopreservation Video Protocol
CryoStor® cell cryopreservation media has been used in the cryopreservation of Plasmodium vivax and P. falciparum sporozoites.
CryoStor, a series of cell-specific, optimized preservation media, is uniquely formulated to address the molecularbiological aspects of cells during the cryopreservation process thereby directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

Other Notes

Formulation contains 2% DMSO.

Legal Information

CryoStor is a registered trademark of BioLife Solutions, Inc.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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A simple and efficient method for cryopreservation and recovery of viable Plasmodium vivax and P. falciparum sporozoites
Singh N, et al.
Parasitology International, 65(5), 552-557 (2016)
Dominic M Clarke et al.
Cytotherapy, 11(4), 472-479 (2009-06-06)
Peripheral blood stem cells (PBSC) have become the preferred stem cell source for autologous hematopoietic transplantation. A critical aspect of this treatment modality is cryopreservation of the stem cell products, which permits temporal separation of the PBSC mobilization/collection phase from
J Stylianou et al.
Cytotherapy, 8(1), 57-61 (2006-04-22)
Hematopoietic stem cells (HSC) have traditionally been frozen using the cryoprotectant DMSO in dextran-40, saline or albumin. However, the process of freezing and thawing results in loss of HSC numbers and/or function. This study investigated the use of CryoStor for

Protocols

CryoStor® Cryopreservation Protocol

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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