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SHH0026

Sigma-Aldrich

PolyFreeze Tissue Freezing Medium

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Synonym(s):
support matrix for tissue sectioning
NACRES:
NA.47

color

clear

application(s)

hematology
histology

storage temp.

room temp

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General description

Tissue samples may be snap frozen using PolyFreeze with isopentane and liquid nitrogen, dry ice (slush/slurry or bunker), or in a cryostat.
PolyFreeze media do not cause autofluorescence and can easily be washed away during tissue fixation when rinsed prior to staining. Once rinsed off the slide, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.
Standard frozen sections at 3–6 μm are easy to obtain and mount on glass slides for drying and/or fixation prior to staining, while thicker sections can be taken as required. Sections will flow freely under an anti-roll device, allowing flat sections to be picked up from the knife-edge. Sections can be either fixed immediately in the fixative of choice or air dried for later fixation and staining. Drying or fixing the slides in the cryostat for specific procedures will not affect the PolyFreeze medium.
um are easy to obtain and mount on glass slides for drying and/or fixation prior to staining, while thicker sections can be taken as required. Sections will flow freely under an anti-roll device, allowing flat sections to be picked up from the knife-edge. Sections can be either fixed immediately in the fixative of choice or air dried for later fixation and staining. Drying or fixing the slides in the cryostat for specific procedures will not affect the PolyFreeze medium.

Application

A support matrix or form of embedding medium for frozen sectioning. This medium freezes quickly supporting the tissue for sectioning at 3 μ and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer.
Cryo-embedding matrix for frozen specimens.
PolyFreeze tissue freezing medium has been used in immunohistochemistry.

Features and Benefits

  • Colored PolyFreeze (blue, green, yellow) makes differentiating multiple specimens easy during sectioning and staining
  • Experience less curling, less ice artifacts, faster freezing with PolyFreeze

Storage and Stability

Store PolyFreeze tissue freezing medium at room temperature. A cloudy appearance of the unfrozen product has no effect on performance.
Store specimens frozen in PolyFreeze medium in liquid nitrogen canisters or in airtight containers in a -80 0C freezer.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

230.0 °F

flash_point_c

> 110 °C


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