F0556
Human Fibronectin
from human fibroblasts, liquid, ~0.5 mg/mL, suitable for cell culture
Synonym(s):
Fibronectin
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About This Item
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product name
Fibronectin solution human fibroblasts, cell culture derived, ~0.5 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
biological source
human fibroblasts
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
31 kDa
packaging
pkg of 100 μL
concentration
~0.5 mg/mL
technique(s)
cell culture | mammalian: suitable
impurities
HBSAG, none detected
HCV, none detected
HIV-1/HIV-2, none detected
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Collagen
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... FN1(2335)
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General description
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.
The matrix protein fibronectin consists of three units that are named Fn1, Fn2 and Fn3. The plasma protein has a molar mass of 31kDa. It is present in loose connective tissue, basement membranes, and the surface of various cell types.
Application
Fibronectin solution from human fibroblasts is used for the following applications:
Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
- It is used in purification of human extracellular matrix (ECM)
- Coating of tissue culture plates with extracellular matrix components
- Used during cell culture (For xeno-free, feeder-free culture the cells were passaged in to human fibronectin along with other components)
Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Biochem/physiol Actions
Fibronectin is a high molecular mass extracellular matrix glycoprotein involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, cell migration/adhesion, and embryogenesis and development. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. It can bind to macromolecules such as collagen, fibrin, glycosaminoglycans, and Clq, apart from some bacteria.It serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts. Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly hence is suitable for tissue engineering applications.
Components
Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.
Caution
The solution should be stored at -20°C. Under these conditions, the product is stable for at least 2 years. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Preparation Note
This product is produced by human fibroblasts and then purified biochemically. It is supplied as a sterile solution at 0.5 mg/mL in a CAPS saline buffer. Before use, the product should be thawed slowly at 2-8°C, forming a clear solution. Avoid any vortexing or vigorous shaking of the solution, which will cause any fibronectin to “crash” out of solution. To coat plates, dilute this product in sterile HBSS solutions and coat with a minimal volume. Then, incubate at 37°C for 1-2 hours and wash 3 times with sterile HBSS and plate cells.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Articles
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocols
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
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