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FC010

Sigma-Aldrich

Human Plasma Fibronectin Purified Protein

This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.

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eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

100
300

sterility

sterile; sterile-filtered

assay

~95% (SDS-PAGE)

form

liquid

mol wt

220 kDa

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

2—10 μg/cm2

input

sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)

UniProt accession no.

binding specificity

Peptide Source: Collagen

Peptide Source: Laminin

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

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This Item
F1056F0162F0895
vibrant-m

F1056

Fibronectin human plasma

vibrant-m

F0895

FIBRONECTIN FROM HUMAN PLASMA

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

100, 300

Quality Level

200

Quality Level

200

Quality Level

200

assay

~95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

-

sterility

sterile; sterile-filtered

sterility

sterile

sterility

-

sterility

sterile

concentration

1 mg/mL

concentration

-

concentration

-

concentration

0.1% (Solution)

General description

Fibronectins (FNs) are high-molecular-mass adhesive glycoproteins of the extracellular matrix (ECM) and body fluids. It is a dimer composed of two subunits linked by two disulfide bonds. Each monomer of FN consists of three types of repeating units: 12 FN repeats of type I (approximately 40 amino acids each), two type II repeats (around 60 amino acids), and 15-17 type III repeats (approximately 90 amino acids). These repeating units contribute to the structural and functional diversity of fibronectin molecules.

Application

Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

Biochem/physiol Actions

Fibronectin plays a crucial role in mediating diverse cellular interactions and is involved in various processes, including cell adhesion, the establishment and maintenance of cell morphology, cell migration, growth, and differentiation. It interacts with several extracellular matrix (ECM) and cell surface proteins, such as collagen, heparin, fibrin, and specific cell membrane receptors. Fibronectin can also serve as a ligand for multiple integrins, including the well-known fibronectin receptor alpha5-beta1, which binds to the arginylglycylaspartic acid (RGD) sequence in the repeat III-10 region of fibronectin. This extensive network of interactions highlights fibronectin′s significance in regulating cellular behavior and mediating tissue function.

Physical form

Liquidin 50 mM tris-buffered saline (TBS), pH 7.5, sterile filtered, containing nopreservatives.

Storage and Stability

Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.

Analysis Note

A double band of 220 kDa is present under reduced conditions.hFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.Plasma from donors has been screened, and shown to be negative for HIV, HTLV, Hepatitis B and C.

Other Notes

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3.Introduce the proper CO2 atmosphere, if required.

4.Add the calculated amount of HFN to each culture vessel.

5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6.Remove residual balanced salt solution before proceeding with standard cell culture procedures

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Aaron T Dirck et al.
Molecular biology of the cell, 31(3), 196-208 (2019-12-19)
The human herpesvirus-7 (HHV-7) U21 glycoprotein binds to class I major histocompatibility complex (MHC) molecules in the endoplasmic reticulum (ER) and reroutes them to lysosomes. How this single viral glycoprotein efficiently redirects the U21/class I MHC complex to the lysosomal
Integrin activation by dithiothreitol or Mn2+ induces a ligand-occupied conformation and exposure of a novel NH2-terminal regulatory site on the beta1 integrin chain.
Ni, H, et al.
The Journal of Biological Chemistry, 273, 7981-7987 (1998)
Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
Seltana, Amira, et al.
Wound Repair and Regeneration, 18, 114-122 null
Sandrine B Lavenus et al.
The Journal of biological chemistry, 295(19), 6700-6709 (2020-04-03)
Tumor cells can spread to distant sites through their ability to switch between mesenchymal and amoeboid (bleb-based) migration. Because of this difference, inhibitors of metastasis must account for each migration mode. However, the role of vimentin in amoeboid migration has
Alternative splicing of the angiogenesis associated extra-domain B of fibronectin regulates the accessibility of the B-C loop of the type III repeat 8
Elisa Ventura, et al.
PLoS ONE, 5(2), e9145-e9145 (2010)

Articles

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Protocols

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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