MilliporeSigma
All Photos(1)

Documents

FIBRP-RO

Roche

Fibronectin (pure)

from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

Sign Into View Organizational & Contract Pricing

Synonym(s):
Fibronectin
MDL number:

biological source

human plasma

Quality Level

sterility

non-sterile; 0.2 μm filtered

assay

>95% (SDS-PAGE)

form

lyophilized (clear, colorless solution after reconstitution)

mol wt

440 kDa

packaging

pkg of 1 mg (11051407001)
pkg of 5 mg (11080938001)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
10838039001F2733MAB88916-C
Roche

FIBRP-RO

Fibronectin (pure)

Fibronectin from human plasma

10838039001

Fibronectin

Fibronectin-FITC FITC-labeled human plasma fibronectin, Suitable for cell culture

F2733

Fibronectin-FITC

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, inhibition assay: suitable, western blot: suitable

biological source

human plasma

biological source

human plasma

biological source

-

biological source

mouse

assay

>95% (SDS-PAGE)

assay

95% (SDS-PAGE)

assay

-

assay

-

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

mol wt

440 kDa

mol wt

440 kDa

mol wt

-

mol wt

-

General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Specificity

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

Sequence

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Physical form

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Preparation Note

Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Roberta Bulla et al.
Nature communications, 7, 10346-10346 (2016-02-03)
Complement C1q is the activator of the classical pathway. However, it is now recognized that C1q can exert functions unrelated to complement activation. Here we show that C1q, but not C4, is expressed in the stroma and vascular endothelium of
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Benjamin N Rollo et al.
F1000Research, 4, 113-113 (2015-06-13)
The avian enteric nervous system (ENS) consists of a vast number of unusually small ganglia compared to other peripheral ganglia. Each ENS ganglion at mid-gestation has a core of neurons and a shell of mesenchymal precursor/glia-like enteric neural crest (ENC)
Fibronectin at a glance.
Roumen Pankov et al.
Journal of cell science, 115(Pt 20), 3861-3863 (2002-09-24)

Articles

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Protocols

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service