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F2518

Sigma-Aldrich

Fibronectin human foreskin fibroblasts

lyophilized powder, suitable for cell culture

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Synonym(s):
Fibronectin
EC Number:
MDL number:
NACRES:
NA.75

biological source

human foreskin fibroblasts

Quality Level

product line

BioReagent

form

lyophilized powder

mol wt

~550 kDa

packaging

pkg of 0.5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

impurities

Human Source, none detected (HCV)
Human Source, none detected (HIV)
Human Source, none detected (HTLV)
Human Source, none detected (HbSAg)
Microbial Contamination, passes test

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

UniProt accession no.

binding specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

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This Item
F0556F2006F0635
Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture

F2006

Fibronectin human plasma

Fibronectin rat plasma powder, BioReagent, suitable for cell culture

F0635

Fibronectin rat plasma

biological source

human foreskin fibroblasts

biological source

human fibroblasts

biological source

human plasma

biological source

rat plasma

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

solution

form

lyophilized powder

form

powder

mol wt

~550 kDa

mol wt

31 kDa

mol wt

450 kDa

mol wt

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Features and Benefits

  • Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
  • It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
  • The starting material is tested and found negative for HIV and HBsAg.
  • The product is soluble in sterile water, yielding a clear, colorless solution.
  • It can be stored at -20°C for at least three years.

Caution

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Physical form

Lyophilized in CAPS buffered saline

Preparation Note

dialyzed against chloroform

Reconstitution

Store reconstituted solution in working aliquots at -20°C or lower.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Fibronectin
Fabrizio M and Angela S
Encyclopedia of Immunology, 909-913 (1998)
Regulation of matrix Accumulation (2012)
Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery.
Tsang T M, et al.
Infection and Immunity, 78(8), 3358-3368 (2010)
Artificial biomaterials for urological tissue engineering
Farhat WA and Geutjes, PJ
Journal of Separation Science (2009)
Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model.
Gursoy U K, et al.
Cytotechnology, 68(6), 2345-2354 (2016)

Articles

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocols

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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