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F6140

Sigma-Aldrich

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse

clone FN-3E2, ascites fluid

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Synonym(s):
Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FN-3E2, monoclonal

mol wt

antigen 240 kDa

contains

15 mM sodium azide

species reactivity

mouse, human, chicken

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:400 using cultured chicken fibroblasts
radioimmunoassay: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

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This Item
F7387F0916SAB4200784
Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

Gene Information

human ... FN1(2335)

Gene Information

human ... FN1(2335)

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

culture supernatant

species reactivity

mouse, human, chicken

species reactivity

mouse, human, chicken

species reactivity

human

species reactivity

chicken, rat, human, mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.

Specificity

The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin.

Immunogen

fibronectin antigen released in culture by a human breast cancer cell line

Application

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in:
  • immunoblot
  • immunofluorescent microscopy
  • immunocytochemistry

Biochem/physiol Actions

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis .
Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mahmut Selman Sakar et al.
Nature communications, 7, 11036-11036 (2016-03-17)
Planar in vitro models have been invaluable tools to identify the mechanical basis of wound closure. Although these models may recapitulate closure dynamics of epithelial cell sheets, they fail to capture how a wounded fibrous tissue rebuilds its 3D architecture.
A Vaheri
Schweizerische medizinische Wochenschrift, 110(40), 1437-1440 (1980-10-04)
Fibronectin is a high molecular weight glycoprotein which is found in a soluble form in plasma (cold-insoluble globulin) and other body fluids and in an insoluble form in connective tissues and associated with basement membranes. Soluble fibronectin interacts with denatured
Lisandra E de Castro Brás et al.
Journal of proteomics, 86, 43-52 (2013-05-18)
The extracellular matrix (ECM) is a critical tissue component, providing structural support as well as important regulatory signaling cues to govern cellular growth, metabolism, and differentiation. The study of ECM proteins, however, is hampered by the low solubility of ECM
ADAMTS-10 and -6 differentially regulate cell-cell junctions and focal adhesions
Cain SA, et al.
Scientific reports, 6 (2016)
Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment
McLane JS, et sl.
Biophysical Journal, 109 (2015)

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