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A5228

Sigma-Aldrich

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-Actin, α-Smooth Muscle, Acta2 Antibody, Alpha Smooth Muscle Actin Antibody Sigma, SMA Antibody - Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, Sma Antibody, alpha SMA, SMA
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

PBS solution

mol wt

antigen ~42 kDa

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable using smooth muscle cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
indirect ELISA: suitable
indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections
microarray: suitable
western blot: suitable using smooth muscle cells

isotype

IgG2a

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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This Item
A2547F3777A7732
conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

1A4, monoclonal

clone

1A4, monoclonal

clone

1A4, monoclonal

clone

EA-53, monoclonal

form

PBS solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

mol wt

-

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).
Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the NH2 terminal synthetic decapeptide of a smooth muscle actin, coupled to keyhole limpet hemocyanin (KLH).
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Application

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal has been used in:
  • immunofluorescence staining
  • immunohistochemistry
  • immunoblotting
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Biochem/physiol Actions

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, cytokinesis, secretion, cytoplasmic streaming and phagocytosis. It has been shown that the relative proportion of actin isoforms are different in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions. The actin in cells of various species and tissue origin are very similar in their immunological and physical properties.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Up-regulation of alpha-smooth muscle actin in cardiomyocytes from non-hypertrophic and non-failing transgenic mouse hearts expressing N-terminal truncated cardiac troponin I
Kern S, et al.
FEBS Open Bio, 4(8), 11-17 (2014)
Danna M Breen et al.
Arteriosclerosis, thrombosis, and vascular biology, 29(7), 1060-1066 (2009-04-11)
Insulin has both growth-promoting and protective vascular effects in vitro, however the predominant effect in vivo is unclear. We investigated the effects of insulin in vivo on neointimal growth after arterial injury. Rats were given subcutaneous control (C) or insulin
Special characteristics of culturing mature human bladder smooth muscle cells on human amniotic membrane as a suitable matrix
Sharifiaghdas F, et al.
Urology, 6(4), 283-288 (2009)
Risk of dissection in thoracic aneurysms associated with mutations of smooth muscle alpha-actin 2 (ACTA2)
Disabella E, et al.
Heart, 97(4), 321-326 (2011)
Jung-Yeon Kim et al.
International journal of molecular medicine, 39(5), 1188-1194 (2017-04-14)
Cholestatic liver disease is characterized by the progressive destruction of biliary epithelial cells (BECs) followed by fibrosis, cirrhosis and liver failure. Activated hepatic stellate cells (HSCs) and portal fibroblasts are the major cellular effectors of enhanced collagen deposition in biliary fibrosis.

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