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SAB4200679

Sigma-Aldrich

Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody produced in mouse

clone 1A4, purified from hybridoma cell culture

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Synonym(s):
AAT6, ACTSA, MYMY5, actin, alpha 2, aorta, smooth muscle
NACRES:
NA.41

biological source

mouse

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

lyophilized powder

mol wt

antigen ~42 kDa

species reactivity

frog, mouse, hamster, chicken, viper, bovine, crayfish, goat, rat, cat, sheep, guinea pig, lizard, human, rabbit, dog, snail

technique(s)

direct ELISA: suitable
immunoblotting: 2-4 μg/mL using extracts of mouse heart tissues
immunohistochemistry: 5-10 μg/mL using human appendix or tonsil tissue sections

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)

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This Item
SAB4200287SAB4200602A5691
conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

-

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

1A4, monoclonal

clone

HHF-35, monoclonal

clone

5C5, monoclonal

clone

1A4, monoclonal

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

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General description

Monoclonal Anti-Actin, α-Smooth Muscle-HRP (mouse IgG2a isotype) is derived from the hybridoma 1A4 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to a sequence of α-smooth muscle actin, conjugated to KLH. Actin is a highly conserved protein, expressed as six isoforms and represents differentiation markers of muscle tissues.

Immunogen

a synthetic peptide corresponding to a sequence at the NH2 terminal synthetic decapeptide of α-smooth muscle actin, conjugated to KLH.

Application

Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody has been used in:
  • direct-immunoblotting
  • direct-immunohistochemistry
  • direct- enzyme linked immunosorbent assay (ELISA)
  • direct staining of tissues and cells
  • characterization of stromal cell heterogeneity in various organs and distinguish smooth muscle cells from fibroblasts in mixed cultures.

Biochem/physiol Actions

Actin and Myosin are two major cytoskeletal proteins implicated in cell motility, both constitutively expressed in many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. It has been shown that relative proportions of actin isoforms are diverse in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions.

Physical form

Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

Preparation Note

Reconstitute in 0.1mL of distilled water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)

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