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SAB5500002

Sigma-Aldrich

Anti-SMA antibody, Rabbit monoclonal

clone SP171, recombinant, expressed in proprietary host, affinity isolated antibody

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NACRES:
NA.41

biological source

rabbit

Quality Level

recombinant

expressed in proprietary host

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

SP171, monoclonal

species reactivity

human (tested)

species reactivity (predicted by homology)

rabbit, rat, bovine, chicken, mouse, pig

technique(s)

immunoblotting: 1:50
immunohistochemistry: 1:200

isotype

IgG

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)

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This Item
A5228F3777A5691
Gene Information

human ... ACTA2(59)

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

Gene Information

mouse ... Acta2(11475)
rat ... Acta2(81633)

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

species reactivity

human (tested)

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

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General description

Smooth muscle actin-α (SMA), also known as α2-smooth muscle actin (ACTA2), is a cytoskeleton protein in smooth muscle cells. It is encoded by the gene mapped to human chromosome 10q23.31. SMA is a vascular smooth muscle specific isoform,
Smooth muscle actin-alpha (SMA) is a cytoskeleton protein in smooth muscle cells and their derived tumors such as leiomyoma and leiomyosarcoma. It is also expressed in myoepithelial cells of the breast and salivary gland, but not in fibroblasts, striated muscle, and myocardium.

Immunogen

Synthetic peptide near the N-terminus of human SMA protein.

Application

Anti-SMA antibody, Rabbit monoclonal has been used in:
  • western blotting
  • immunohistochemistry
  • immunofluorescence

Biochem/physiol Actions

Smooth muscle actin-α (SMA)/ α2-smooth muscle actin (ACTA2) interacts with β-myosin heavy chain and facilitates vascular smooth muscle cell contraction. The encoded protein regulates c-MET (tyrosine-protein kinase Met) and focal adhesion kinase (FAK) expression in human lung adenocarcinoma cells, which positively and selectively mediates tumor progression. Thus, SMA can be used as a potential prognostic biomarker and/or target for treating metastatic lung adenocarcinoma. Mutation in the gene is associated with the development of patent ductus arteriosus (PDA), bicuspid aortic valve (BAV), iris flocculi, livedo reticularis, cerebrovascular accident (CVA) and stenosis of the aortic vasa vasorum. In addition, variation in the gene expression leads to thoracic aortic aneurysms and dissections (TAAD).

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Identification of EML4-ALK as an alternative fusion gene in epithelioid inflammatory myofibroblastic sarcoma.
Jiang Q, et al.
Orphanet Journal of Rare Diseases, 12(1), 97-97 (2017)
BBC3 in macrophages promoted pulmonary fibrosis development through inducing autophagy during silicosis.
Liu H, et al.
Cell Death & Disease, 8(3), e2657-e2657 (2017)
Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy.
Liu H, et al.
Particle and Fibre Toxicology, 13(1), 55-55 (2016)
Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells.
Shenoy A K, et al.
The Journal of Clinical Investigation, 126(11), 4174-4186 (2016)
Ying-Chun Zhu et al.
International journal of molecular medicine, 40(4), 1165-1171 (2017-08-30)
Transforming growth factor-β (TGF-β) induces epithelial-mesenchymal transition (EMT) primarily via a Smad‑dependent mechanism. However, there are few studies available on TGF-β-induced EMT through the activation of non‑canonical pathways. In this study, the Cdc42-interacting protein-4 (CIP4)/partitioning-defective protein 6 (Par6) pathway was investigated in TGF-β1‑stimulated NRK-52E cells. Rat

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