MilliporeSigma
All Photos(5)

Documents

F0916

Sigma-Aldrich

Monoclonal Anti-Fibronectin antibody produced in mouse

clone IST-4, ascites fluid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

IST-4, monoclonal

mol wt

antigen 240 kDa

contains

15 mM sodium azide

species reactivity

human

should not react with

rabbit, mouse, goat, bovine, canine, chicken

technique(s)

immunocytochemistry: 1:100 using cultured human fibroblasts
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: 1:250 using human fibroblasts cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
F0791F7387F6140
Gene Information

human ... FN1(2335)

Gene Information

human ... FN1(2335)

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

clone

IST-4, monoclonal

clone

IST-3, monoclonal

clone

FN-15, monoclonal

clone

FN-3E2, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

human

species reactivity

goat, canine, human, chicken, sheep, rabbit, bovine

species reactivity

mouse, human, chicken

species reactivity

mouse, human, chicken

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN′s play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells.
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.
Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody recognizes an epitope located within the 5th type III repeat of human plasma fibronectin. This epitope is common to all fibronectin forms. The antibody localizes plasma and cellular fibronectin both in its native and denatured-reduced forms (240 kDa) applying immunoblotting, RIA, ELISA, immunocytochemistry and immunohistochemistry. The product reacts with human fibronectin. No reactivity is observed with other species including goat, bovine, dog, rabbit, mouse and chicken.

Immunogen

human plasma fibronectin

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Fibronectin antibody has been used :
  • in immunohistochemistry
  • in confocal microscopy
  • in immunocytochemistry
  • western blotting analysis

Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody may be used for the specific labeling of fibronectin in cultured cells and in frozen tissue sections. Staining of formalin-fixed, paraffin-embedded sections may require prolonged proteolytic unmasking (e.g., by pepsin but not by trypsin).

Biochem/physiol Actions

Fibronectin participates in growth, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in the formation of extracellular matrix.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway
Lei Q Y, et al.
Molecular and cellular biology, 28(7), 2426-2436 (2008)
Clinicopathology of graft detachment after D escemet's membrane endothelial keratoplasty
Brockmann T, et al.
Acta Ophthalmologica, 92(7), e556-e561 (2014)
Francesca Di Modugno et al.
Oncogene, 37(42), 5605-5617 (2018-06-17)
We demonstrated previously that the splicing of the actin regulator, hMENA, generates two alternatively expressed isoforms, hMENA11a and hMENAΔv6, which have opposite functions in cell invasiveness. Their mechanisms of action have remained unclear. Here we report two major findings: (i)
The formation of a functional retinal pigment epithelium occurs on porous polytetrafluoroethylene substrates independently of the surface chemistry
Kearns V R, et al.
Journal of Materials Science. Materials in Medicine, 28(8), 124-124 (2017)
Shanna A Arnold et al.
Clinical & experimental metastasis, 33(1), 29-44 (2015-10-13)
The extracellular matrix protein fibronectin (FN) contributes to the structural integrity of tissues as well as the adhesive and migratory functions of cells. While FN is abundantly expressed in adult tissues, the expression of several alternatively spliced FN isoforms is

Protocols

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service