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F4759

Sigma-Aldrich

Fibronectin bovine plasma

powder, BioReagent, suitable for cell culture

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Synonym(s):
Fibronectin
EC Number:
MDL number:
eCl@ss:
42020141
NACRES:
NA.75

biological source

bovine plasma

Quality Level

sterility

sterile

product line

BioReagent

form

powder

mol wt

~450 kDa

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

≥1 mg protein/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

bovine ... FN1(2807940)

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This Item
F2006F0162F0287
vibrant-m

F4759

Fibronectin bovine plasma

vibrant-m

F2006

Fibronectin human plasma

biological source

bovine plasma

biological source

human plasma

biological source

human plasma

biological source

human plasma

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

concentration

≥1 mg protein/mL

concentration

>1 mg/mL protein

concentration

-

concentration

-

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

ambient

General description

Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.
Fibronectins are adhesive glycoproteins and are found both as cell surface proteins and in plasma. Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Application

Fibronectin from bovine plasma is used for the following applications:
  • Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
  • Cell movement assays (culture plates were coated with fibronectin)
  • Cell spreading and immunofluorescence
  • Protein specificity
  • Integration of sensor films into microfluidic chips
  • Histological and Immunohistochemical (IHC) analyses
  • Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.Study shows that human dental pulp (HDP) cells rely on fibronectin (FN) for initial attachment and subsequent spreading. FN may exhibit mitogenic activity, promoting cell proliferation, and also play a role in regulating the differentiation process of pulp cells into odontoblasts. The presence of FN appears to influence the fate and development of HDP cells, specifically in their differentiation into odontoblasts, which are responsible for dentin formation in teeth.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Actions of bovine plasma fibronectin on cultured human dental pulp cells
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Articles

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocols

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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