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C-28077

Sigma-Aldrich

Cancer Cell Line Medium XF

250 ml

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form

liquid

packaging

pkg of 250 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

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C-28075SCC100SCM045
packaging

pkg of 250 ml

packaging

pkg of 250 ml

packaging

-

packaging

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

dry ice

General description

Cancer Cell Line Medium XF

Application

Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability. The medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.

Quality

All lots of the PromoCell Cancer Cell Line Medium XF are subjected to comprehensive quality control tests using a human cancer cell line. Each lot of PromoCell Cancer Cell Line Medium XF is tested for the ability to support proliferation and serial passage of MCF7 breast carcinoma cells on fibronectincoated dishes. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 2 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 2 to 8°C. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 2 to 8°C.

Subculture Routine

Click here for more information.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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Zhikai Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(4) (2022-01-21)
Cancer immunotherapy frequently fails because most carcinomas have few T cells, suggesting that cancers can suppress T cell infiltration. Here, we show that cancer cells of human pancreatic ductal adenocarcinoma (PDA), colorectal cancer, and breast cancer are coated with transglutaminase-2

Related Content

Defined culture conditions. Consistent growth of human cancer cell lines in PromoCell’s serum-free/xeno-free Cancer Cell Line Media-XF. Explore over 350 PromoCell products on Sigma.com

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