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A549 Cell Line human

from human lung(carcinoma), 86012804

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Synonym(s):
A-549 Cells, A549 Cells, NCI-A549 Cells

biological source

human lung (carcinoma)

growth mode

Adherent

karyotype

Hypotriploid

morphology

Epithelial

products

Lecithin, High amounts of de-saturated fatty acids.

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian lung carcinoma

Cell Line Description

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Application

Enzymatic studies
Human A549 cell line has been used:
  • to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
  • to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
  • to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Culture Medium

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or tTrypsin/EDTA; 5% CO2; 37°C.

Other Notes

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