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CC076

Sigma-Aldrich

Human Collagen Type IV

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eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

Assay

95% (Human collagen type IV, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<5% Human collagen types I-III, V, VI, and non-collagen proteins

input

sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

solubility

water: soluble at 20 °C

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COL4A1(1282)

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This Item
C6745C8374C5533
Sigma-Aldrich

Sigma-Aldrich

CC076

Human Collagen Type IV

assay

95% (Human collagen type IV, SDS-PAGE)

assay

-

assay

>85% (SDS-PAGE)

assay

-

form

liquid

form

solution

form

powder

form

powder

concentration

1 mg/mL

concentration

0.3 mg/mL

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

impurities

<5% Human collagen types I-III, V, VI, and non-collagen proteins

impurities

-

impurities

HIV, hepatitis B and hepatitis C, none detected

impurities

HIV, hepatitis B and hepatitis C, none detected

General description

Collagen type IV is the major structural element of basal membranes. Human collagen type IV is a native triple helix. Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type IV was purified by using differential salt precipitation. Purity and retention of native helical structure was controlled by SDS-PAGE and by reaction with anti-collagen type specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL
Product Source: Human placenta, negative for HBsAg and HIV antibodies

Application

Coating material for cell culture studies

Formation of collagen gels

Optimal working dilutions must be determined by end user.

Physical form

Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative.

Storage and Stability

Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

Isolation and partial characterization of a new human collagen with an extended triple-helical structural domain.
Bentz, H, et al.
Proceedings of the National Academy of Sciences of the USA, 80, 3168-3172 (1983)
Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
Sage, H and Bornstein, P
Biochemistry, 18, 3815-3822 (1979)
R W Glanville et al.
European journal of biochemistry, 95(2), 383-389 (1979-04-02)
Native type IV collagen was isolated from human placenta using pepsin solubilisation followed by fractional salt precipitation and chromatogarphic purification. The native preparation was characterised using amino acid analyses, disc gel electrophoresis, segment-long-spacing crystallites and immunological methods. Two component alpha

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