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A-005-M

Sigma-Aldrich

Poly-L-Lysine Solution

0.01%

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Synonym(s):
Lysine
eCl@ss:
32160801
NACRES:
NA.75

needle size

2.0 mm

Quality Level

sterility

sterile

form

liquid

mol wt

Mw 84000 Da

manufacturer/tradename

NovaSeptum®

concentration

0.01 % (w/v)
0.1 mg/mL

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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Poly-L-Lysine Solution

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Poly-D-Lysine solution, 1.0 mg/mL

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Poly-L-Ornithine Solution (0.01%)

vibrant-m

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Poly-L-lysine hydrobromide

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

concentration

0.01 % (w/v), 0.1 mg/mL

concentration

-

concentration

0.01 % (w/v), 0.1 mg/mL

concentration

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

form

liquid

form

liquid

form

liquid

form

lyophilized powder

mol wt

Mw 84000 Da

mol wt

-

mol wt

Mw 78000 Da

mol wt

30,000-70,000

General description

Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. Certain cell types secrete proteases, which can digest Poly-L-Lysine.
The molecular weight of Poly-L-Lysine for cell culture can vary significantly, with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (greater than 300,000 Da) having more binding sites per molecule. This product uses a Poly-L-Lysine of 84000 Da, yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Application

Poly-L-Lysine Solution has been used to coat culture dishes to culture U2-OS cells and primary cortical neurons.

Biochem/physiol Actions

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Quality

Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.

Preparation Note

Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

1. Thaw Poly-L-Lysine solution at room temperature.

2. Dilute Poly-L-Lysine solution to the desired concentration in sterile water.

3. Fully coat the cell culture surface with diluted Poly-L-Lysine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.

4. Allow the cell culture vessel to sit at room temperature overnight.

5. Aspirate the Poly-L-Lysine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein.

Legal Information

NOVASEPTUM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Srsf1 and Elavl1 act antagonistically on neuronal fate choice in the developing neocortex by controlling TrkC receptor isoform expression
A Ioana Weber, et al.
Nucleic Acids Research, 10218-10237 (2023)
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner, et al.
PLoS ONE, 17(7), e0260173-e0260173 (2022)
Polylysine for skin regeneration: A review of recent advances and future perspectives
Zarrintaj P, et al.
Bioengineering & translational medicine, 5;7(1) (2022)
Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Viv
Heo JS, et al.
BioMed Research International (2016)
Ana Popović et al.
Journal of cell science, 136(5) (2023-03-03)
Myosin-X (MYO10), a molecular motor localizing to filopodia, is thought to transport various cargo to filopodia tips, modulating filopodia function. However, only a few MYO10 cargoes have been described. Here, using GFP-Trap and BioID approaches combined with mass spectrometry, we

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Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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