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R6750

Sigma-Aldrich

Ribonucleic acid from baker's yeast (S. cerevisiae)

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Synonym(s):
RNA
CAS Number:
MDL number:
NACRES:
NA.51

Quality Level

storage temp.

−20°C

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This Item
R6625R6875R5636
vibrant-m

R6625

Ribonucleic acid from torula yeast

vibrant-m

R6875

Ribonucleic acid from torula yeast

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).

Application

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.

Preparation Note

Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Customers Also Viewed

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Kazuya Shirato et al.
Virology journal, 11, 139-139 (2014-08-12)
The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets
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Inflammatory Bowel Disease--comprised of Crohn's Disease and Ulcerative Colitis (UC)--is a complex, multi-factorial inflammatory disorder of the gastrointestinal tract. In this study we have explored the utility of naturally occurring circulating miRNAs as potential blood-based biomarkers for non-invasive prediction of
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
Douglas A Weigent
Cellular immunology, 282(2), 71-78 (2013-06-19)
In the present study, we show that higher levels of lymphocyte GH are expressed in spleen cells from aging animals compared to young animals. Further, leukocytes from primary and secondary immune tissues and splenic T and B cells from aging

Protocols

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

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