R6513
Ribonuclease A from bovine pancreas
for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Recommended Products
grade
for molecular biology
Quality Level
form
lyophilized
specific activity
≥70 Kunitz units/mg protein
mol wt
13.7 kDa
~13,700
foreign activity
Exonuclease and endonuclease, none detected
NICKase and DNase, none detected
storage temp.
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
- for plasmid DNA purification
- to digest and avoid staining of double-stranded RNA during staining of nuclei
- in immunoperoxidase and immunofluorescence detection
- to treat cells prior to flow cytometry
- as a component of post-hybridization buffer for washing FFPE tissue sections post in-situ hybridization
- to treat cells during the preparation of outer membrane protein (OMP) extracts
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
Features and Benefits
Analysis Note
Other Notes
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Application
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Protocols
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Related Content
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Chromatograms
application for HPLCOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service