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LIG1

Sigma-Aldrich

DNA Ligation Kit

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NACRES:
NA.53

grade

for molecular biology

Quality Level

usage

 kit sufficient for 150 ligation reactions

shipped in

wet ice

storage temp.

−20°C

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This Item
RDLIG-RO70852DNALIG-RO
Sigma-Aldrich

Sigma-Aldrich

LIG1

DNA Ligation Kit

SpinPrep Gel DNA Kit Rapid, efficient extraction of DNA from agarose gels

Millipore

70852

SpinPrep Gel DNA Kit

T4 DNA Ligase solution, from Escherichia coli(NM 989), suitable for activity assay

Roche

DNALIG-RO

T4 DNA Ligase

usage

 kit sufficient for 150 ligation reactions

usage

sufficient for 160 reactions (04898125001), sufficient for 40 reactions (04898117001)

usage

-

usage

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

10-30°C

storage temp.

−20°C

General description

Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions. Sticky-end ligations are more efficient than blunt-end ligations; these can be facilitated with the addition of PEG.

Application

Suitable for:
  • Joining fragments of DNA into a cloning vector
  • Mutagenesis
  • Gene anyalysis and structure-function relationships

Components

Sufficient for 150 reactions:
  • 300uL 10X Ligation Buffer (D2176) in 250 mM Tris-HCl (pH 7.8) 100 mM MgCl2, and 10 mM dithiothreitol
  • 3 x 100 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM dithiothreitol
  • 3 x 100 uL 10 mM ATP ( A3702)
  • 50 uL Control pBR322 DNA, HAE III Digest (D9430) 0.5 ug/ul in 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA
  • 1.5 mL 24% (w/v) PEG Solution, (P 2454)
  • 1.5 mL Molecular Biology Grade Water (W4502)

Principle

One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sambrook, J., et al
Molecular Cloning: A Laboratory Manual, 2, 1-1 (1989)
Lei Wei et al.
Nature microbiology, 5(5), 715-726 (2020-03-11)
Chronic hepatitis B virus (HBV) infections result in 887,000 deaths annually. The central challenge in curing HBV is eradication of the stable covalently closed circular DNA (cccDNA) form of the viral genome, which is formed by the repair of lesion-bearing
Ausubel, F. M.
Current Protocols in Molecular Biology, 1, 3-3 (1994)
Quanxin Long et al.
PLoS pathogens, 13(12), e1006784-e1006784 (2017-12-30)
Hepadnavirus covalently closed circular (ccc) DNA is the bona fide viral transcription template, which plays a pivotal role in viral infection and persistence. Upon infection, the non-replicative cccDNA is converted from the incoming and de novo synthesized viral genomic relaxed
Bradley J Eckelmann et al.
NAR cancer, 2(3), zcaa013-zcaa013 (2020-08-11)
Homologous recombination/end joining (HR/HEJ)-deficient cancers with BRCA mutations utilize alternative DNA double-strand break repair pathways, particularly alternative non-homologous end joining or microhomology-mediated end joining (alt-EJ/MMEJ) during S and G2 cell cycle phases. Depletion of alt-EJ factors, including XRCC1, PARP1 and

Protocols

The cloning process requires the ligation of linear DNA into a cloning vector. This ability to join fragments of DNA through recombinant technology is essential for many basic experiments in biotechnology.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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