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D7291

Sigma-Aldrich

Deoxyribonuclease I RNase-free solution from bovine pancreas

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Synonym(s):
DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.53

biological source

bovine pancreas

Quality Level

grade

for molecular biology

form

buffered aqueous glycerol solution

specific activity

10000 units/mg protein





mol wt

29.1 kDa

UniProt accession no.

foreign activity

RNase, none detected

storage temp.

−20°C

Gene Information

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This Item
D4263DN25R4642
grade

for molecular biology

grade

-

grade

-

grade

for molecular biology

form

buffered aqueous glycerol solution

form

powder

form

lyophilized powder

form

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

specific activity

10000 units/mg protein





specific activity

-

specific activity

≥400 Kunitz units/mg protein

specific activity

-

mol wt

29.1 kDa

mol wt

~31 kDa

mol wt

~31 kDa

mol wt

13.7 kDa, ~13,700

UniProt accession no.

P00639

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Components

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Unit Definition

One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Other Notes

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

Protocols

Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.

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