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11579681001

Roche

RNase, DNase-free, High Concentration

from bovine pancreas

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Synonym(s):
Rnase

biological source

bovine pancreas

Quality Level

form

solution

specific activity

≥30 U/mg

packaging

pkg of 1 mg (10 mg/ml)

manufacturer/tradename

Roche

concentration

10 mg/mL

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This Item
R6148AMPD110109134001
Sigma-Aldrich

Sigma-Aldrich

R6148

RNase A solution

DNase I Amplification Grade

Sigma-Aldrich

AMPD1

DNase I

RNase from bovine pancreas

Roche

10109134001

RNase

form

solution

form

liquid

form

liquid

form

dry powder (prepared from crystallized ribonuclease)

specific activity

≥30 U/mg

specific activity

1500 units/mL, 3000 units/mL

specific activity

-

specific activity

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

concentration

10 mg/mL

concentration

20 mg/mL in Tris buffer

concentration

1 unit/μL

concentration

-

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

100

General description

A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.

Application

High concentration, DNase-free RNase has been used for:
  • Genomic DNA isolation
  • Isolation of plasmid DNA
  • Degradation of RNA for flow cytometry

Quality

Absence of DNase activity tested according to the current Quality Control procedures.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Preparation Note

Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0

Other Notes

For life science research only. Not for use in diagnostic procedures.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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