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RNASEA-RO

Roche

RNase A

from bovine pancreas

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Synonym(s):
Rnase
Enzyme Commission number:

biological source

bovine pancreas

Quality Level

form

dry powder

specific activity

50 U/mg (+25°C, RNA as substrate)

mol wt

Mr = 13,700

packaging

pkg of 100 mg (10109169001)
pkg of 25 mg (10109142001)

manufacturer/tradename

Roche

parameter

65 °C optimum reaction temp.

technique(s)

flow cytometry: suitable

optimum pH

7.0-7.5

storage temp.

2-8°C

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This Item
101091340011078635700110104159001
RNase A from bovine pancreas

Roche

RNASEA-RO

RNase A

RNase from bovine pancreas

Roche

10109134001

RNase

Ribonuclease H (RNase H) from Escherichia coli H 560 pol A1

Roche

10786357001

Ribonuclease H (RNase H)

DNase I grade II, from bovine pancreas

Roche

10104159001

DNase I

form

dry powder

form

dry powder (prepared from crystallized ribonuclease)

form

solution

form

lyophilized

specific activity

50 U/mg (+25°C, RNA as substrate)

specific activity

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

specific activity

~40000 units/mg protein

specific activity

-

mol wt

Mr = 13,700

mol wt

-

mol wt

-

mol wt

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

technique(s)

flow cytometry: suitable

technique(s)

-

technique(s)

cDNA synthesis: suitable

technique(s)

DNA extraction: suitable

General description

Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.

Application

  • For analytical purposes
  • Isolation of DNA (for this purpose, RNase A should be boiled)
  • For cell cycle analysis by flow cytometry and propidium iodide (PI) staining

Biochem/physiol Actions

RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Reconstitution

RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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