70856
RNase A Solution
Convenient solution for selective degradation of RNA
Sign Into View Organizational & Contract Pricing
All Photos(1)
Recommended Products
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
10 mg/mL
technique(s)
DNA purification: suitable
shipped in
ambient
storage temp.
10-30°C
General description
RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.
Application
RNase A Solution has been used:
- in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
- in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
- to treat control adult and embryonic thymi cells during staining
- to remove RNA from affibody–mRNA fusion clones before clone screening
Biochem/physiol Actions
RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.
Warning
Toxicity: Standard Handling (A)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Myc controls a distinct transcriptional program in fetal thymic epithelial cells that determines thymus growth
Nature Communications (2019)
Neurotrophic properties and the de novo peptide sequencing of edible bird's nest extracts
Food Bioscience, 32, 100466-100466 (2019)
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(11), 2681-2692 (2020-01-16)
Malignancy of cancer cells depends on the active transcription of tumor-associated genes. Recently, unique clusters of transcriptional enhancers, termed super-enhancers, have been reported to drive the expression of genes that define cell identity. In this study, we characterized specific super-enhancer-associated
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service