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RNase A Solution

Convenient solution for selective degradation of RNA

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UNSPSC Code:
12352202
NACRES:
NA.54

form

solution

manufacturer/tradename

Novagen®

storage condition

OK to freeze

concentration

10 mg/mL

technique(s)

DNA purification: suitable

shipped in

ambient

storage temp.

10-30°C

General description

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

Application

RNase A Solution has been used:
  • in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
  • in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
  • to treat control adult and embryonic thymi cells during staining
  • to remove RNA from affibody–mRNA fusion clones before clone screening

Biochem/physiol Actions

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

wgk_germany

WGK 2


Certificates of Analysis (COA)

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