R5500
Ribonuclease A from bovine pancreas
Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein
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biological source
bovine pancreas
Quality Level
type
Type XII-A
assay
≥90% (SDS-PAGE)
form
lyophilized powder
specific activity
75-125 Kunitz units/mg protein
mol wt
~13,700
technique(s)
cell based assay: suitable
impurities
salt, essentially free
suitability
suitable for mRNA or total RNA extracted from cells and tissues
application(s)
diagnostic assay manufacturing
foreign activity
protease, essentially free
storage temp.
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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General description
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
Biochem/physiol Actions
Features and Benefits
Preparation Note
Analysis Note
Application
inhibitor
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Protocols
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Chromatograms
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