Skip to Content
MilliporeSigma
All Photos(1)

Documents

796-0658

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
Proteins, mixture

description

Separated on Discovery® BIO PolyMA-SCX

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase: [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
detector: UV, 280 nm

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

standard

Product No.
Description
Pricing

C4011

Cytochrome c from pigeon breast muscle, ≥95% based on Mol. Wt. 12,173 basis

C4879

α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder

C2037

Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis

C3131

Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

C7150

Cytochrome c from equine heart, suitable for GFC marker, BioReagent

C7752

Cytochrome c from equine heart, ≥95% based on Mol. Wt. 12,384 basis

C2506

Cytochrome c from equine heart, ≥95% (SDS-PAGE)

C2436

Cytochrome c from Saccharomyces cerevisiae, ≥85% based on Mol. Wt. 12,588 basis

R5125

Ribonuclease A from bovine pancreas, Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

R5000

Ribonuclease A from bovine pancreas, Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

R6513

Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

R5500

Ribonuclease A from bovine pancreas, Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

R4875

Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

R5250

Ribonuclease A from bovine pancreas, Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

R5503

Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein

C4129

α-Chymotrypsin from bovine pancreas, Type II, lyophilized powder, ≥40 units/mg protein

C3483

Cytochrome c from human heart, ≥95% (SDS-PAGE), lyophilized powder

30398

Cytochrome c from bovine heart, ≥95% (GE)

C2867

Cytochrome c from equine heart, BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service