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DN25

Sigma-Aldrich

Deoxyribonuclease I from bovine pancreas

lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein

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Synonym(s):
Deoxyribonuclease, DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

form

lyophilized powder

Quality Level

specific activity

≥400 Kunitz units/mg protein

mol wt

~31 kDa

composition

Protein, ≥85%

solubility

0.15 M NaCl: soluble 5.0 mg/mL, hazy

application(s)

diagnostic assay manufacturing
diagnostic assay manufacturing

foreign activity

RNase ≤0.02%

shipped in

wet ice

storage temp.

−20°C

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1 of 4

This Item
D5025D4513D4263
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

solubility

0.15 M NaCl: soluble 5.0 mg/mL, hazy

solubility

0.15 M NaCl: soluble 5.0 mg/mL, clear, colorless

solubility

0.15 M NaCl: soluble 5.0 mg/mL, clear(lit.)

solubility

0.15 M NaCl: soluble 5.0 mg/mL, clear

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.
  • Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
  • Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
  • DNase I structure resembles the structure of to exonuclease III. It includes two central ß sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]

Application

  • Decreases viscosity providing better yields by removing DNA in primary cell isolation:
  • Incorporating labelled bases into DNA: DNA nick
  • Radioactive labelling
  • Bioprocessing applications: DNA removal
  • Eliminating genomic DNA from RNA preparations before RT-PCR
  • In vitro transcription
  • Nick translation
  • DNase footprinting
  • Actin regulation of actin polymerization in cells, and cell apoptosis
  • UV crosslinking of proteins to nucleic acids
  • DNase play a role in the regulation of actin polymerization in cells and is involved in apoptosis process [1]
Used for the removal of DNA from protein samples.

Biochem/physiol Actions

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Features and Benefits

Our Deoxyribonuclease DNAse I,is essentially RNAse free product, which support the product application.

Unit Definition

One Kunitz unit will produce a change in A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III, as substrate.

Physical form

Crude preparation, contains calcium chloride

Preparation Note

10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.

Analysis Note

Protein determined by biuret.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Purification of rat spermatogenic cells and preliminary biochemical analysis of these cells.
M L Meistrich et al.
Biology of reproduction, 25(5), 1065-1077 (1981-12-01)
G A Scarborough
Proceedings of the National Academy of Sciences of the United States of America, 73(5), 1485-1488 (1976-05-01)
Biochemicalical evidence is presented which demonstrates that the Neurospora crassa plasma membrane ATPase (ATP phosphohydrolase, EC 3.6.1.3) is an electrogenic pump. The electrical potential across the Neurospora plasma membrane, as monitored by [14C]SCN- uptake by isolated Neurospora plasma membrane vesicles
Comparison of the multiple forms of bovine pancreatic deoxyribonuclease.
J Salnikow et al.
The Journal of biological chemistry, 245(21), 5685-5690 (1970-11-10)
Enzymes of Molecular Biology
Weir, A.F.
Methods in Molecular Biology, 16(2), 2-16 null
Ling-Shiang Chuang et al.
Gastroenterology, 151(4), 710-723 (2016-07-06)
Crohn's disease (CD) has the highest prevalence in Ashkenazi Jewish populations. We sought to identify rare, CD-associated frameshift variants of high functional and statistical effects. We performed exome sequencing and array-based genotype analyses of 1477 Ashkenazi Jewish individuals with CD

Protocols

To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.

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