MilliporeSigma
All Photos(1)

Documents

LIBTM-RO

Roche

Liberase TM Research Grade

medium Thermolysin concentration

Sign Into View Organizational & Contract Pricing

Quality Level

form

lyophilized

packaging

pkg of 10 mg (05401119001 [2 x 5 mg])
pkg of 100 mg (05401127001 [2 x 50 mg])

manufacturer/tradename

Roche

parameter

35-37 °C optimum reaction temp.

technique(s)

single cell analysis: suitable
tissue processing: suitable

color

white

optimum pH

7.4

solubility

water: soluble

suitability

suitable for flow cytometry

application(s)

life science and biopharma
sample preparation

foreign activity

Endotoxin 1 EU/mg

shipped in

dry ice

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
LIBTH-ROLIBDH-ROCOLLP-RO
Liberase™ TM Research Grade medium Thermolysin concentration

LIBTM-RO

Liberase TM Research Grade

Liberase™ TH Research Grade lyophilized, suitable for tissue processing, optimum pH 7.4

LIBTH-RO

Liberase TH Research Grade

Liberase™ DH Research Grade lyophilized, optimum pH 7.4, optimum reaction temp. 35-37 °C

LIBDH-RO

Liberase DH Research Grade

Collagenase P from Clostridium histolyticum

COLLP-RO

Collagenase P

application(s)

life science and biopharma
sample preparation

application(s)

-

application(s)

life science and biopharma

application(s)

life science and biopharma

suitability

suitable for flow cytometry

suitability

-

suitability

-

suitability

-

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

optimum pH

7.4

optimum pH

7.4

optimum pH

7.4

optimum pH

-

General description

Liberase TM Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a medium concentration of Thermolysin, a non-clostridial neutral protease.

Application

Liberase(Thermolysin Medium) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.

Features and Benefits

  • Maximize viability and yield of isolated cells: with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
  • Count on higher specific activity of the enzyme blend: as a result of higher Collagenase I + II purity (determined by HPLC analysis).
  • Obtain higher experimental reproducibility: due to higher lot-to-lot consistency.
  • Increase safety: with an enzyme that is free of any mammalian or avian tissue-derived raw materials.

Preparation Note

Stabilizers: Calcium
Working concentration: Liberase Research Grade Enzyme Working Concentration

Liberase enzymes have significantly higher specific activities than traditional collagenases. This means that the working concentration of Liberase Research Grade Purified Enzymes, expressed in mg/ml, will be lower than that of traditional collagenase.
When the application is not included on this list, first use Liberase TM Research Grade at a concentration of 0.08–0.28 Wünsch units/ml.The goal is to determine the best starting concentration of Liberase Research Grade Enzyme Blends. This is a starting point, and the final concentration may vary due to differences in procedure and lot-to-lot differences in traditional collagenase.

Collagenase Working Concentration

Multiply your previous collagenase working concentration (mg/ml) by its specific activity (Wünsch units/mg, [as determined above]), to obtain Wünsch units/ml. To determine how much Liberase Research Grade Enzyme Blend to use, first multiply your collagenase working concentration (in Wünsch units/ml) times the total volume of your working enzyme solution to obtain the total collagenase activity needed (Wünsch units). Divide the total collagenase activity required by the Liberase Research Grade stock concentration ( “Reconstitution and Storage”). This indicates how many milliliters of Liberase Research Grade Enzyme Blend stock solution to use in your working enzyme solution.
Storage conditions (working solution): Store unused stock solution in single-use aliquots at -15 to -25 °C.

Note: Avoid repeated freezing and thawing!

Reconstitution

Reconstitute the lyophilized enzyme with injection-quality sterile water or tissue-dissociation buffer. Do not add serum or other components, such as albumin or protease inhibitors, to the dissociation buffer. Enzyme stability is reduced at higher concentrations and warmer temperatures (4 °C). Avoid both the above conditions.

Reconstitute the entire vial. Do not weigh individual aliquots of the lyophilizate. The introduction of moisture into the vial results in a decline in enzymatic activity.

Place vial on ice to rehydrate the lyophilized enzyme.

Gently agitate the vial at 2 to 8 °C until enzyme is completely dissolved (max. 30 min).

Depending on the type of tissue-dissociation buffer used to dissolve Liberase Research Grade Purified Enzyme Blends, slight precipitations may be observed which readily dissolve in the dilutedworking solution, and have no influence on enzyme activity.

Remove an aliquot of the stock solution to prepare the working solution.

Reconstitution volume

2 ml (1 vial with 5 mg–10 mg pack size), 10 ml (1 vial with 50 mg–100 mg pack size)

Collagenase Wünsch (units/ml)

13 (1 vial with 5 mg–10 mg pack size), 26 (1 vial with 50 mg–100 mg pack size)

Total Collagenase concentration [mg/ml]

2.5 (1 vial with 5 mg–10 mg pack size), 5.0 (1 vial with 50 mg–100 mg pack size)

Storage and Stability

Keep container tightly closed in a dry and well-ventilated place.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Liberase is a trademark of Roche

also commonly purchased with this product

Product No.
Description
Pricing

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Camilo Riquelme-Guzmán et al.
Open biology, 12(6), 220078-220078 (2022-06-22)
In processes such as development and regeneration, where large cellular and tissue rearrangements occur, cell fate and behaviour are strongly influenced by tissue mechanics. While most well-established tools probing mechanical properties require an invasive sample preparation, confocal Brillouin microscopy captures
Steven M Blaauboer et al.
eLife, 4, doi:10-doi:10 (2015-04-22)
Effective mucosal adjuvants enhance the magnitude and quality of the vaccine response. Cyclic di-GMP (CDG) is a promising mucosal vaccine adjuvant. However, its in vivo mechanisms are unclear. Here, we showed, in mice, that CDG elicits stronger Ab and TH
Luca F R Gebert et al.
Nucleic acids research, 42(1), 609-621 (2013-09-27)
MicroRNAs (miRNAs) are short noncoding RNAs, which bind to messenger RNAs and regulate protein expression. The biosynthesis of miRNAs includes two precursors, a primary miRNA transcript (pri-miRNA) and a shorter pre-miRNA, both of which carry a common stem-loop bearing the
William Tyler Frantz et al.
eLife, 12 (2023-04-07)
Tissue-resident stem and progenitor cells are present in many adult organs, where they are important for organ homeostasis and repair in response to injury. However, the signals that activate these cells and the mechanisms governing how these cells renew or
Aaron M Hosios et al.
Nature metabolism, 4(12), 1792-1811 (2022-12-20)
The mechanistic target of rapamycin complex 1 (mTORC1) senses and relays environmental signals from growth factors and nutrients to metabolic networks and adaptive cellular systems to control the synthesis and breakdown of macromolecules; however, beyond inducing de novo lipid synthesis

Protocols

Liberase™ TM Research Grade Protocol & Troubleshooting

Related Content

Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service