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E4378

Sigma-Aldrich

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

≥97.0%

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Synonym(s):
EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
Linear Formula:
[-CH2OCH2CH2N(CH2CO2H)2]2
CAS Number:
Molecular Weight:
380.35
Beilstein/REAXYS Number:
1717370
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

assay

≥97.0%

form

powder

reaction suitability

reagent type: chelator

loss

≤1.0% loss on drying

mp

241 °C (dec.) (lit.)

solubility

1 M NaOH: 1.1 g/10 mL, clear, colorless

storage temp.

room temp

SMILES string

OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

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1 of 4

This Item
E039603777324626
EGTA, Molecular Biology Grade

324626

EGTA, Molecular Biology Grade

-
Quality Level

400

Quality Level

200

Quality Level

300

Quality Level

100

mp

241 °C (dec.) (lit.)

mp

241 °C (dec.) (lit.)

mp

241 °C (dec.) (lit.)

mp

-

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

10-30°C

solubility

1 M NaOH: 1.1 g/10 mL, clear, colorless

solubility

1 M NaOH: 0.1 M, clear, colorless, H2O: soluble (increases as pH is increased)

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

solubility

1 M NaOH: soluble

form

powder

form

powder

form

powder

form

solid (fine)

Application

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used as a component of:
  • ice-cold cardioplegia solution for transverse aortic constriction in rats
  • ice-cold lysis buffer for protein extraction and whole extract cells
  • buffer A, B and, C for the extraction of mitochondria from HEK293F cells

A chelating agent useful for the determination of calcium in the presence of magnesium.

Biochem/physiol Actions

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Physical properties

solubility in water increases with pH

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)


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