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E8145

Sigma-Aldrich

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥97%

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Synonym(s):
EGTA tetrasodium, Egtazic acid tetrasodium, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium
Empirical Formula (Hill Notation):
C14H20N2O10Na4
CAS Number:
Molecular Weight:
468.28
MDL number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

assay

≥97%

solubility

H2O: soluble 10%, clear, colorless

SMILES string

[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCOCCOCCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4

InChI key

BAOGCDPNNOBWQZ-UHFFFAOYSA-J

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This Item
E437814513E0396
Quality Level

200

Quality Level

400

Quality Level

200

Quality Level

200

solubility

H2O: soluble 10%, clear, colorless

solubility

1 M NaOH: 1.1 g/10 mL, clear, colorless

solubility

H2O: 10 mg/mL, clear, faintly yellow

solubility

1 M NaOH: 0.1 M, clear, colorless, H2O: soluble (increases as pH is increased)

Application

A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca2+.
EGTA is suitable for use:
  • in media for chelating Ca2+ to facilitate efficient incorporation of Sr2+ into the oocytes
  • as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins
  • as a component of oocyte activation media

Biochem/physiol Actions

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).
Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca2+ in the presence of Mg2+ at a ratio of 1:1.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic reprogramming research and gene manipulation in animals. Although the success rates of somatic cloning have been inefficient and the mechanism of reprogramming is still largely unknown
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Zhang C, et al.
acta societatis botanicorum poloniae, 85(3) (2016)
Schmid, R.W. et al.
Analytical Chemistry, 29, 264-264 (1957)
EGTA treatment of human airways in vitro unmasks M1/MUC5AC mucin in submucosal glands
Roger P, et al.
The European Respiratory Journal, 18(1), 176-183 (2001)

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