E8145

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥97%

• Synonym(s): EGTA tetrasodium, Egtazic acid tetrasodium, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium
• Empirical Formula (Hill Notation): C₁₄H₂₀N₂O₁₀Na₄
• CAS Number: 13368-13-3
• Molecular Weight: 468.28
• MDL number: MFCD00274420
• UNSPSC Code: 12352202
• PubChem Substance ID: 24894667
• NACRES: NA.77

Properties

• assay: ≥97%
• technique(s): RNA purification: suitable
• solubility: H₂O: soluble 10%, clear, colorless
• application(s): life science and biopharma
• SMILES string: [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCOCCOCCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4
• InChI key: BAOGCDPNNOBWQZ-UHFFFAOYSA-J

Description

Application

A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca²⁺.

EGTA is suitable for use:

• in media for chelating Ca²⁺ to facilitate efficient incorporation of Sr²⁺ into the oocytes
• as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins
• as a component of oocyte activation media

Biochem/physiol Actions

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).

Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca²⁺ in the presence of Mg²⁺ at a ratio of 1:1.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)