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HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2) application for HPLC

Materials

standard

Product No.
Description
Pricing

Cytochrome c from pigeon breast muscle

≥95% based on Mol. Wt. 12,173 basis

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

Cytochrome c from equine heart

suitable for GFC marker, BioReagent

Cytochrome c from equine heart

≥95% based on Mol. Wt. 12,384 basis

Cytochrome c from equine heart

≥95% (SDS-PAGE)

Cytochrome c from Saccharomyces cerevisiae

≥85% based on Mol. Wt. 12,588 basis

Ribonuclease A from bovine pancreas

Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

Ribonuclease A from bovine pancreas

Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

Ribonuclease A from bovine pancreas

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Ribonuclease A from bovine pancreas

Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

Ribonuclease A from bovine pancreas

Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

Ribonuclease A from bovine pancreas

Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

Ribonuclease A from bovine pancreas

Type I-AS, 50-100 Kunitz units/mg protein

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

Cytochrome c from human heart

≥95% (SDS-PAGE), lyophilized powder

Cytochrome c from bovine heart

≥95% (GE)

Cytochrome c from equine heart

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

CONDITIONS

column

Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)

mobile phase

[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)

flow rate

0.5 mL/min

injection

10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

detector

UV, 280 nm

Description

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany