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10786357001

Roche

Ribonuclease H (RNase H)

from Escherichia coli H 560 pol A1

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Synonym(s):
rnase h

Quality Level

form

solution

specific activity

~40000 units/mg protein

packaging

pkg of 100 U

manufacturer/tradename

Roche

optimum pH

7.5-9.1

shipped in

dry ice

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This Item
R651311119915001RNASEA-RO
Ribonuclease H (RNase H) from Escherichia coli H 560 pol A1

Roche

10786357001

Ribonuclease H (RNase H)

RNase, DNase-free from bovine pancreas

Roche

11119915001

RNase, DNase-free

RNase A from bovine pancreas

Roche

RNASEA-RO

RNase A

specific activity

~40000 units/mg protein

specific activity

≥70 Kunitz units/mg protein

specific activity

≥30 units/mg protein

specific activity

50 U/mg (+25°C, RNA as substrate)

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

optimum pH

7.5-9.1

optimum pH

-

optimum pH

-

optimum pH

7.0-7.5

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

100

General description

Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.

Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.
Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.

Application

Ribonuclease H (RNase H) has been used for:
  • In vivo RNA-primed initiation of DNA synthesis
  • Elimination of mRNA during second-strand cDNA synthesis
  • Site-specific cleavage of RNA
  • Detection of RNA:DNA regions in double-stranded DNA of natural origin
  • Removal of poly (A) sequences of mRNA if oligo (dT) is present
  • RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)

Biochem/physiol Actions

Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Using RNase H for degrading mRNA results in 80% depletion of mRNA and protein expression. RNase H recognizes the start codon and the 3′ and 5′ untranslated regions. This enzyme participates in DNA replication.

Features and Benefits

  • Eliminate potential sources of PCR errors.
  • Increase accessibility of primers during subsequent PCR.

Quality

Absence of endonucleases, nicking activities, and ribonucleases.

Unit Definition

RNase H is assayed according to Hillenbrand and Staudenbauer. One unit of RNase H is the amount of enzyme which produces 1 nmol acid-soluble ribonucleotides from[3H] poly(A) x poly(dT) in 20 minutes at +37 °C under the stated assay conditions.

Volume Activity: Approximately 1 U/μl

Preparation Note

Activator: The enzyme has its maximal activity in presence of SH-reagents

Storage and Stability

Store at -15–-25 °C. (unopened)

Other Notes

For life science research only. Not for use in diagnostic procedures. Using RNase H after the cDNA synthesis step can increase the sensitivity of a two-step RT-PCR assay.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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