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Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications.

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grade

Molecular Biology

Quality Level

Assay

≥99% phenol basis (titration)
>99% chloroform basis (HPLC)

form

liquid (Two-layer)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

colorless

shipped in

ambient

storage temp.

2-8°C

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This Item
25668220201574793
Chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Sigma-Aldrich

25668

Chloroform – isoamyl alcohol mixture

Premium Grade
assay

≥99% phenol basis (titration), >99% chloroform basis (HPLC)

assay

≥99.5% (chloroform + isoamyl alcohol, GC)

assay

≥98% (NMR)

assay

-

form

liquid (Two-layer)

form

liquid

form

crystalline powder

form

liquid

Quality Level

100

Quality Level

200

Quality Level

300

Quality Level

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

storage condition

-

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze, desiccated

General description

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.
A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Prior to equilibration with the Tris-based equilibration buffer (pH ~6.7), the mix is ideal for extraction of proteins and lipids for RNA purification. Following equilibration with the Tris-based equilibration buffer (pH ~8.0), is ideal for extraction of proteins and lipids for DNA purification. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.

Warning

Toxicity: Toxic (F)

Reconstitution

Following equilibration, refrigerate (4°C).

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


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