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C0549

Sigma-Aldrich

Chloroform:Isoamyl alcohol 24:1

suitable for nucleic acid purification

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Synonym(s):
chloroform-isoamyl alcohol
NACRES:
NA.52

form

liquid

Quality Level

contains

0.6-1.0% ethanol as stabilizer

suitability

suitable for nucleic acid purification

application(s)

agriculture

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459844320021AX1440
Ethyl alcohol, Pure 200 proof, ACS reagent, ≥99.5%

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459844

Ethyl alcohol, Pure

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3-Methyl-1-butanol ACS reagent, ≥98.5%

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Essential Grade
Isoamyl Alcohol Meets ACS Specifications GR ACS

Supelco

AX1440

Isoamyl Alcohol

-
application(s)

agriculture

application(s)

agriculture

application(s)

-

application(s)

-

Quality Level

200

Quality Level

100, 200

Quality Level

200

Quality Level

200

contains

0.6-1.0% ethanol as stabilizer

contains

-

contains

-

contains

-

suitability

suitable for nucleic acid purification

suitability

-

suitability

-

suitability

-

General description

Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.

Application

Chloroform:Isoamyl alcohol 24:1 has been used:

  • to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.
  • for a single DNA precipitation step for high-quality DNA extraction.
  • for protein extraction and RNAse treatment.
  • in phenolic oxidation to separate phenolics from the aqueous portion.
  • for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).
  • in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C


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A modified CTAB-based protocol for total RNA extraction from the medicinal plant Impatiens balsamina (Balsaminaceae) for next-generation sequencing studies.
FOONG LC, et al.
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Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
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Optimisation of DNA extraction from the crustacean Daphnia.
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PeerJ, 4, e2004-e2004 (2016)

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