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P2069

Sigma-Aldrich

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA

Supplied with Equilibration buffer, for molecular biology

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Synonym(s):
DNA extraction
NACRES:
NA.52

grade

for molecular biology

Assay

≥99.0%

form

liquid

pH

6.5-6.9(pH of phenolic phase)
7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

application(s)

agriculture

storage temp.

2-8°C

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This Item
P3803P1944N3910
assay

≥99.0%

assay

≥99.0%

assay

≥99.0%

assay

-

form

liquid

form

liquid

form

liquid

form

liquid

pH

6.5-6.9(pH of phenolic phase), 7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

pH

6.5-6.9(pH of phenolic phase)

pH

4.3-4.7

pH

-

application(s)

agriculture

application(s)

agriculture

application(s)

life science and biopharma

application(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

For applications requiring a higher pH, such as the isolation of large intact genomic DNA, addition of the Equilibration Buffer is recommended.
Phenol:Chloroform:Isoamyl Alcohol has been used in the preparation of RNA for the study of total RNA isolation from myocardial biopsies. It has been also used to extract DNA during Human Androgen Receptor (HUMARA) Clonality Assay.

Packaging

Supplied with P3803 and a separate bottle of equilibration buffer to adjust the phenol phase to pH 8.0 ± 0.2.

Suitability

This lot of phenol:chloroform:isoamyl alcohol 25:24:1 was use tested by extracting a crude preparation of pBR322 DNA preparation from E. coli. Based on evaluation by agarose gel electrophoresis the extracted DNA was not degraded or reduced in concentration. The same procedure was applied to purified pBR322 DNA and lambda Hind III digest DNA. Based on electrophoretic evaluation the purified preparations were not detectably degraded or reduced in concentration.

Preparation Note

Add the entire contents of the small bottle of Equilibration Buffer to the large bottle of Phenol:Chloroform:Isoamyl Alcohol. Mix gently and allow the phases to separate before use, approximately 2-4 hours. This will adjust the pH of the Phenol solution from pH 6.7 ± 0.2 to 8.0 ± 0.2.

Other Notes

After the addition of Equilibration Buffer, the product is stable for 6 months vs. 2 years without added buffer. Storage at -20 °C protected from light extends the shelf life to greater than 1 year after the addition of Equilibration Buffer.

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

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